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Image Search Results
Journal: Human Reproduction Open
Article Title: Human iPSCs derived from cryopreserved testicular somatic cells enable germline regeneration in childhood cancer survivors
doi: 10.1093/hropen/hoag054
Figure Lengend Snippet: Overview of the experimental layout: focus on primary testicular somatic cell cultures and reprogramming . ( A ) Schematic overview of the experimental procedures. ( B ) Non-genome-integrating, feeder-free reprogramming of human induced pluripotent stem cells (hiPSCs) from primary testicular somatic cells, with the corresponding time required for each step. Left panel: Phase-contrast images of confluent primary testicular somatic cell cultures approximately 1 week after isolation from the cryopreserved tissue; Middle panel: Emerging hiPSC colonies following the mRNA-based reprogramming protocol; Right panel: Expansion of the isolated hiPSC clones NFKI001 at passage 8 (p8) and NFKI002 at p15 from Patients 1 and 2, respectively. Scale bars, 500 μm. Figure (A) was created using BioRender (Alves Lopes, J. P. (2026); https://BioRender.com/gv6k171 ).
Article Snippet: Upon reaching confluency, cells were seeded at 2000 cells/cm 2 in 35 mm dishes on day-3 and transfected twice daily from day 0 to day 4 using the
Techniques: Isolation, Clone Assay